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Climate Geoengineering – The Secret Spraying Of Our Skies

Source: Project Censored For decades now, and largely in secret, the US military-industrial complex has been engaged in the systematic “spraying” of large swaths of our sky in one of the largest and least-discussed global

Breaking News: Air Raids, Chemical Weapons Hit California

Since at least the 1990′s, chemical weapons have been used in a spray form against the American people from the air, on a more or less continuous basis.  The attacks continue today.  The planes are

Geoengineering Watch Global Alert News, November 24, 2018, #172

Dane Wigington GeoengineeringWatch.org Civilization is sinking in a rapidly rising sea of lies, deception and criminality. How many are fully focused on holiday dinners, parades and football games, even as the great unraveling of our paradigm

The Gulf of Mexico Is Still Dying: A Special Update On The BP Gulf Oil Spill

Pathogenic Micro-organisms Proliferate Due To Polluted And Poisoned GOM ‘Bioterrain’ Source: State of the Nation There have been several significant developments over the past few decades in the Gulf of Mexico (GOM) which now require

Organic agriculture ravaged by chemtrails – Monsanto seizes the opportunity, profits and dominates

Organic farmers have yet another environmental hazard to contend with, this time compliments of the U.S. government in the form of chemtrails. A mess of toxic chemicals, these harmful sprays pollute the soil, water and

Arctic Ice Sixth Lowest In At Least A Thousand Years

The more people get over their disdain for Al Gore and begin to do honest investigation, the more they will be able to recognize well spun propaganda from Big Oil And The Geoengineers. Arctic ice is plummeting

“Rain of terror: Egypt to crack down on ‘fake’ weather reports”

Dane Wigington GeoengineeringWatch.org The unfolding planetary climate collapse is accelerating by the day with covert global climate engineering operations further fueling the overall process. In an ever more desperate attempt to hide the climate engineering assault

Geoengineering and Ozone Destruction

Again it seems appropriate to wonder what will it take for the gravity of the aerosol spraying to be realized by a larger percentage of the population. What it will take to compel more people

CYMATICS: Science Vs. Music – Nigel Stanford

Chemtrails: Frightening Lesser-Known Facts

"In the last ten years, respiratory disease in the US has moved from 8th to 3rd highest cause of death. Asthma rates have more than doubled in the western world and Alzheimer's' disease, a condition

The HAARP Storms

There is a live time event taking place this April 13th, which is an impossibility and yet it is being generated by unnatural manipulations to the weather system. This evening at 11 PM eastern time,

Video: The Vietnam War And The Phoenix Program: “A Computerized Genocide”

Source: Global Research Michael Maclear’s 1975 documentary, Spooks and Cowboys, Gooks and Grunts (Part 1) Introduction by Douglas Valentine Michael Maclear’s 1975 documentary, Spooks and Cowboys, Gooks and Grunts (Part 1) is more relevant now

Duty To Warn: Changing The “War No More” Sentiment Of Armistice Day To The War-Glorifying

Duty To Warn Changing the “War No More” Sentiment of Armistice Day to the War-Glorifying Propaganda of Veterans Day By Gary G. Kohls, MD 11-11-17 99 years ago, on 11-11-1918, at precisely 11 am Paris

Weekly Geoengineering Watch Update 1-26-2014

Thy Wayseer Manifesto

ATTENTION: All you rule-breakers, you misfits & troublemakers, all you free-spirits & pioneers... Everything the establishment has told you is wrong with you - is actually what's right with you.

Study Shows Radiofrequency Radiation Impact on Aspen Seedlings, International Journal of Forestry Research

Aspens have been rapidly dying throughout Colorado since 2004. The attached article, “Adverse Influence of Radio Frequency Background on Trembling Aspen Seedlings: Preliminary Observations”, published in the International Journal of Forestry Research (Volume 2010 (2010),

Geoengineering Watch Global Alert News, August 3, 2024, #469

Dane Wigington GeoengineeringWatch.org "Scientists Study This Very Scary Climate Strategy" (from Mother Jones Journal). "Introducing particles like sulfate aerosols into the stratosphere could create a plethora of new and unpredictable problems. Possible negative impacts may include changing regional

Dane Wigington, “GeoEngineering: Man Playing God”

My most sincere thanks to Eric Gajewski and TradCatKnight Radio for helping to sound the alarm on the critical climate engineering issue.

Attention U.S. Military Personnel! Did You Know You Swore an Oath to Defend a FOREIGN Private Corporation (United States, Inc.)?

Source: omnithought.org If you are in the U.S. military and care about freedom and life, you need to be aware that you swore an oath to defend the Constitution of the United States, Inc., a

Geoengineering Watch Global Alert News, July 3, 2021, #308

Dane Wigington GeoengineeringWatch.org Record heat, fires, smoke, hurricanes, floods and huge hail, how’s is your 4th of July holiday going? Western North America is yet again being baked and burned to the ground, and summer is

Behind the Geoengineering Curtain

Warming Oceans, Warming Planet, The Bottom Line. Dane Wigington geoengineeringwatch.org Taking The Bait The planet is in full blown meltdown and even now the spin masters have successfully triggered a new "global cooling" mob. It

More Mass Failure Of Geoengineering, Ocean Fertilization Can’t Work

Dane Wigington geoengineeringwatch.org Man's war with nature is ultimately a war against himself. How many "interventions gone wrong" will have to occur before the lesson is finally learned by the human race? Should it come as

Higher Elevations Are Warming Too, And Faster

Source: Radio Canada Int'l For some time studies have indicated that polar regions, especially the Arctic, is warming at a faster rate than those regions closer to the equator. A new international study appears to

Dismantling The Lie Of “Contrails”

Those in power have done their absolute best to convince an all too gullible public that our constantly hazed out skies are just the result of "contrails", this is an absolute lie. All those that

The Balancing Act

New studies: ‘Conspiracy theorists’ sane; government dupes crazy, hostile

In short, the new study by Wood and Douglas suggests that the negative stereotype of the conspiracy theorist – a hostile fanatic wedded to the truth of his own fringe theory – accurately describes the

Geoengineering Watch Global Alert News, May 27, 2017

Dane Wigington GeoengineeringWatch.org Convenient catastrophes continue to provide the power structure the premise they need to fan the flames of the public's fears. The Manchester suicide bombing is the latest tragedy that paved the way for

“Anonymous” Covers Climate Engineering Threat

The group "Anonymous" is constantly and actively working toward exposing tyranny in countless arenas around the globe. The video below addresses the most critical issue of global climate engineering, it was assembled by Anonymous and

Climate Engineering Toxic Fallout And Northeastern US Tree Die Off

Trees are dying at an astounding rate all over the globe. Even in areas that are getting plenty of rain the die off continues to accelerate. This short video is an excellent documentation of the

Climate Engineering, Runaway Climate Collapse, Dead Judges, And The Push Toward WWlll, What’s The Connection

Dane Wigington geoengineeringwatch.org Connecting the dots involves standing back far enough to examine the bigger picture, all are a part of the picture. End games are being played out on many fronts as the walls close in from

Graphene Skies?

Dane Wigington
GeoengineeringWatch.org

What aren’t we being told? Is the highly toxic and controversial element graphene being seeded into our skies as part of the ongoing covert climate intervention operations? Is climate modification the only motive behind the elements being utilized for atmospheric aerosol spraying programs? Are other agendas also being carried out? Do the weather makers and their controllers consider the consequences of their actions? Or is it possible that many of the consequences are, in fact, part of the agenda? Please review the attached 5 minute video report for input and answers.

All are needed in the critical battle to wake populations to what is coming, we must make every day count. Share credible data from a credible source, make your voice heard. Awareness raising efforts can be carried out from your own home computer.
DW

Must view, THE DIMMING, our most comprehensive climate engineering documentary:​

Must view, THE DIMMING, our most comprehensive climate engineering documentary:​

For Geoengineering Watch awareness materials shown below, click the image to order.

      GW flyer packet 300   GeoengineeringWatch.org booklets, DVD

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34 Responses

  1. In B.C Canada in 2021 we had what they called an atmospheric river. I always thought something was not rite. I watched the dimming a few months ago and learned even more than I thought they where doing. Dane was talking about the so called 5 g microwave towers. I was wondering if they where using graphine with geo engineering. Now I'm not an expert , but in my thought wave that would make the rain a conductor and there for weponizing those towers to do bad things with the rain like …….. just sayin.

    1. A "graphene sky" is reference to prophecies of Habakkuk. So the wealthy, lunatic controllers can sweep over the nations like locusts, stealing up all their "wealth". I.e., privacy and lives.

  2. They are so relentlessly spraying over Germany these days ,there are over 20chemtrail lines over my house in North-Rhine Westfalen. Pure Horror.

    1. Central Virginia gets heavily inundated with jet Chemtrails almost every single day. They must have made a modification to the technology because these new "clouds" are looking rather rippled and waveform-like, if that makes any sense.

  3. I've only known about chemtrails since about 2008 and I've tried to tell people about it while most look at me like I have two heads. 
    If we only have until 2025, then I want to spend as much time as I can in church and with those I love the most!'

  4. Absolutely correct.You have public science papers about advantages of graphene oxide in jet fuels.Want to report you that in eastern Europe they are spraying visible mash on our skies in last few years.Its getting worse.Incredible summer storms.Trees dying. Last days we have oddly clear blue sky.Why? Satanist are busy with war prepares over Ukraine.Resistance!

  5. Numerous chemtrails have been injected into the Forest Lake, Minnesota skies nearly daily for the past year. I've also observed chemtrails on a regular basis over Coon Rapids, MN. I've taken hundreds of photos to document these trails and the odd cloud formations that follow them. 

  6. I noticed the alarming plaid layering of trails in the skys about a week before Christmas….a week later "Omicron" is rampant in our area… Hmmm interesting….

  7. Dane, why don't you get in touch with the likes of David Attenborough and Greta thunberg – please. People need a leader, someone who has reputation, someone who is already widely known. That is our only option to spread te message across the world. 

    1. Hello, Hope, thank you for standing with us in this all important battle. Yes, I agree that such well known individuals need to get a heads up so that they start to look up. I have reached out to as many as possible and had Geoengineering Watch materials delivered to many, but I confess that I need all the help I can get with this task. I will continue to tend as many fires as I can in the battle, but there is just not enough time in a day for me. If we have any chance of reaching a critical mass of awareness in time, it will take our collective efforts, all of us.

      https://dev.geoengineeringwatch.org/how-do-we-stop-climate-engineering-only-way-forward/

       

    2. David A. will not speak on this matter and Greta is entirely a puppet of the false Global Warming scare-mongering campaign. Only ordinary people can stop this dangerous geoengineering when enough numbers wake up to the reality.

      The massive rainfall and floods in Sydney Australia this week was also preceded by spraying activity clearly visible for over a week. Now over 50,000 houses evacuated…

       

    3. Hope, Attenborough and Thunberg are BOTH members of the WEF and NWO. They spout about climate change all the time….when the truth is this….our skies have been the target of spraying since 1932 when Leningrad started experimenting…
       

  8. my sister lives in a mexican village border with arizona, yuma, and she is scared now because her body is completely magnetized like those pics we see of the vaccinated people though she is far from getting the jab!  coins, cell phone, everything metal glues to her skin!!!

  9. Its time we studied what makes human beings want to harm others.   Our approach seems to be still compulsion – behaviour modification – when we know that each child needs to develop its unique self and learn to respect the selves of others.   Compulsion only makes people angry – or deviously cooperative from fear of retaliation – like school children.   Learning freedom with responsibility is the only way, but it has to have a foundation of meeting infancy needs for a mother – a known affectionate person who is always there.   Innocence deprived of its entitlement becomes a diabolical spirit.

     

    1. VERY precise and ABSOLUTELY COHERENT comment that goes to the ROOT of a culture where people are willing to harm others in having been raised parked in front of televisions and screens in absolute deprivation of the relational requirements of a developing neurology.

    2. We are studying that. These people are sociopaths, psychopaths and narcissists. There is no cure; just stop glorifying them and choosing them to run things.

  10. raymond

    you want to move – necessities

    spring water, lots of wild plants not south facing – too hot these days

    susan ferguson thank you for so much info

     

  11. Geoengineering as conveyed in this documentary, is without doubt a blatant and appalling Act of War against The People, The Planet and All Life as we know it!   

  12. US used Graphene net which is an exceptional conductor and is a strategic weapon requiring Presidential Sign Off against Baghdad in 2002 as the entire city went dark and the fragments shorted out all attempts to bring electricity back to the city for years as only spots could be rebuilt since all of the infrastructure was not possible to clear or clean.  It is 1 atom wide and created by DARPA and MIT in large sheets.  The term to tear this netting is called fission.

    To bad that this is not known except for the few of us that monitored this event.  This will end up in the trash like all the rest.  The US is not what you think it is or was.  Owned by the Crown, the Temple Knights of St. John in the City of London Corporation of which the Inner temple and middle temple still exist.  

    Frederick Gates was the lead researcher from the Carnegie School, now Rockefeller University that created the extremely deadly Pneumonia, Meningitis "Vaccine" in 1918.  I have the paper.

    1818-1824 it was Cholera that these Saturn worshippers let loose in India and west Asia for UK and US for the Opium Wars to come.

     

  13. VSF: This is only one company of many in the graphene business. There are five others that are recommended as stock-buys for investors. Applied Graphene Materials (AGM), Haydale Graphene Industries (HAYD), AIXTRON (AIXA), Applied Graphene Materials (AGM), ZEN Graphene Solutions (ZEN).

     

    The Future of Graphene and 5G
    Grolltex Manager, April 2nd, 2018

    At the February Mobile World Congress, held in Barcelona, Spain, the close connection between graphene and 5G technology was one of the main sources of attraction. Both have been featured prominently in the news recently as new-age wonders, so it’s only natural that the two would at some point combine forces to produce some truly remarkable capabilities. That time appears to be in the very near future, as considerable research has been underway on ways that the two could support each other for mutual benefit.

    Monolayer graphene has only been commercially available for about the last decade, but it has already made a powerful impact in the areas of high-speed photosensitivity, farming applications, flexible photodetectors, medical diagnostics, and water purification processes. 5G of course, has been hailed as the future of communications for several years, and is considered to be the enabling technology for both VR and AR. Some of the ongoing research has been related to finding ways that super-conductive, flexible monolayer graphene could be used to support the needs of 5G technology, to help finally achieve the breakthrough it needs.

    Graphene and 5G Technology Combined

    5G technology cannot simply be scaled up from previous technology to meet the demands of high-speed communications of the future – it needs an enabling technology. Enter monolayer graphene. By late 2017, a research team at Chalmers University in Sweden had developed a method of combining graphene flexibility with terahertz detection so as to make it possible to connect the Internet of Things (IoT), via high-bandwidth technologies available in 5G.

    Another group of researchers comprised of Italian University specialists and commercial companies, have produced a flexible microwave device for communications which has applications for Wi-Fi, and which can also be used in millimeter wave (mmWave) technologies. And why is this important? It’s universally acknowledged that the true future of 5G technology lies in this millimeter wave technology.

    Currently some of the 5G high-frequency components are made using gallium arsenide technology, but this is very costly, and has been one of the factors slowing the development of 5G. Graphene can be manufactured much more inexpensively, and its quality is at least comparable and often superior to gallium arsenide. The only thing which hinders the shift from gallium arsenide to graphene is the immaturity of the graphene supply chain – this is a technology barely 10 years old, and there are not a lot of manufacturers able to supply components in the quantity that would be needed.

    The Future of Graphene

    The versatility of graphene will become more and more evident in the next several years, as graphene will be used in the manufacture of a wide range of superior products such as water desalination membranes, medical diagnostic devices, smartphone displays, stretchable electronics, advanced solar cells, and others. Graphene is clearly the wave of the future, and it will be fascinating to observe its ongoing development.

    Grolltex is the most advanced graphene producer in the U.S. Through patented transfer and processing methods, we are able to produce single layer graphene at a cost structure not previously known. There are currently more than 15,000 products under patent-pending status which are based on graphene technology, and that number will most assuredly be growing in the coming few years. To acquire high-quality graphene sheets for sale, or monolayer graphene, contact us with any questions you might have, or to place an order.

    https://grolltex.com/the-future-of-graphene-and-5g/

  14.     •    08 May 2020
    Graphene oxide-incorporated hydrogels for biomedical applications
        •    Jongdarm Yi,
        •    Goeun Choe,
        •    Junggeon Park &
        •    Jae Young Lee
    Polymer Journal volume 52, pages 823–837 (2020)

    Abstract
    Graphene and graphene derivatives (e.g., graphene oxide (GO)) have been incorporated into hydrogels to improve the properties (e.g., mechanical strength) of conventional hydrogels and/or develop new functions (e.g., electrical conductivity and drug loading/delivery). Unique molecular interactions between graphene derivatives and various small or macromolecules enable the fabrication of various functional hydrogels appropriate for different biomedical applications. In this mini-review, we highlight the recent progress in GO-incorporated hydrogels for biomedical applications while focusing on their specific uses as mechanically strong materials, electrically conductive scaffolds/electrodes, and high-performance drug delivery vehicles.

    https://www.nature.com/articles/s41428-020-0350-9

  15. Properties of Graphene Oxide

    One of the advantages of the gaphene oxide is its easy dispersability in water and other organic solvents, as well as in different matrixes, due to the presence of the oxygen functionalities. This remains as a very important property when mixing the material with ceramic or polymer matrixes when trying to improve their electrical and mechanical properties.
    On the other hand, in terms of electrical conductivity, graphene oxide is often described as an electrical insulator, due to the disruption of its sp2 bonding networks. In order to recover the honeycomb hexagonal lattice, and with it the electrical conductivity, the reduction of the graphene oxide has to be achieved. It has to be taken into account that once most of the oxygen groups are removed, the reduced graphene oxide obtained is more difficult to disperse due to its tendency to create aggregates.
    Functionalization of graphene oxide can fundamentally change graphene oxide’s properties. The resulting chemically modified graphenes could then potentially become much more adaptable for a lot of applications. There are many ways in which graphene oxide can be functionalized, depending on the desired application. For optoelectronics, biodevices or as a drug-delivery material, for example, it is possible to substitute amines for the organic covalent functionalization of graphene to increase the dispersability of chemically modified graphenes in organic solvents. It has also been proved that porphyrin-functionalized primary amines and fullerene-functionalized secondary amines could be attached to graphene oxide platelets, ultimately increasing nonlinear optical performance.
    In order for graphene oxide to be usable as an intermediary in the creation of monolayer or few-layer graphene sheets, it is important to develop an oxidization and reduction process that is able to separate individual carbon layers and then isolate them without modifying their structure. So far, while the chemical reduction of graphene oxide is currently seen as the most suitable method of mass production of graphene, it has been difficult for scientists to complete the task of producing graphene sheets of the same quality as mechanical exfoliation, for example, but on a much larger scale. Once this issue is overcome, we can expect to see graphene become much more widely used in commercial and industrial applications.

    https://www.graphenea.com/pages/graphene-oxide#.YN9U3x1lCGg

  16. Nanomaterials-Based Electrochemical Immunosensors
    by Zhenguo Zhang, Yulin Cong, Yichun Huang and Xin Du *

    College of Life Sciences, Key Laboratory of Food Nutrition and Safety, Shandong Normal University, Jinan 250014, China

    Abstract
    With the development of nanomaterials and sensor technology, nanomaterials-based electrochemical immunosensors have been widely employed in various fields. Nanomaterials for electrode modification are emerging one after another in order to improve the performance of electrochemical immunosensors. When compared with traditional detection methods, electrochemical immunosensors have the advantages of simplicity, real-time analysis, high sensitivity, miniaturization, rapid detection time, and low cost. Here, we summarize recent developments in electrochemical immunosensors based on nanomaterials, including carbon nanomaterials, metal nanomaterials, and quantum dots. Additionally, we discuss research challenges and future prospects for this field of study. View

    https://www.mdpi.com/2072-666X/10/6/397

  17. Fabrication and Characterization of a Biomaterial Based on Extracellular-Vesicle Functionalized Graphene Oxide
    Julia Driscoll, Anuradha Moirangthem, Irene K. Yan and Tushar Patel*
        •    Department of Transplantation, Mayo Clinic, Jacksonville, FL, United States

    Mesenchymal stem cell (MSC) derived extracellular vesicles (EV) are emerging as acellular therapeutics for solid organ injury and as carriers for drug delivery. Graphene-based materials are novel two-dimensional crystal structure-based materials with unique characteristics of stiffness, strength and elasticity that are being explored for various structural and biological applications. We fabricated a biomaterial that would capture desirable properties of both graphene and stem cell derived EV.

    Developmental Toxicity of GO-EV Treatment
    To determine if GO and sGO-based biomaterials have in vivo effects, we evaluated their toxicity using zebrafish. Dechorionation and hatching in zebrafish takes place between 48 and 72 hpf. First, we monitored the hatching rate of the zebrafish starting at 45 hpf. In comparison to vehicle treated control zebrafish, there was a modest delay in the hatching rates of the zebrafish treated with 100 μg/mLGO and GO-EV, conversely, the rates in zebrafish treated with 10 μg/mL EV were accelerated (Figure 7). A similar acceleration in hatching was observed in the zebrafish treated with 10 μg/mL sGO, whereas the zebrafish treated with 10 μg/mL sGO-EV, 100 μg/mL sGO and 100 μg/mL sGO-EV all exhibited delayed hatching rates (Supplementary Figure 1). Next, we evaluated for the development of any malformations. In zebrafish exposed to GO-EV, yolk sac edema and pericardial edema were observed. Pericardial edema was also observed in the zebrafish treated with either low or high concentrations of sGO-EV. On the contrary, there were no malformations observed in the zebrafish treated with PBS, GO, or sGO at either 10 or 100 μg/mL. The heart rate varied considerably at different time points, but we did not observe any trends toward decreased heart rate in any treatment groups (data not shown). We further assessed survival of zebrafish at 168 hpf. A slight increase in mortality was observed in zebrafish treated with GO-EV, and with higher concentrations of sGO or sGO-EV but there was no lethality observed in zebrafish treated with PBS, GO or lower concentrations of sGO.
    Discussion
    In the present study we have developed a graphene oxide-based biomaterial synthesized by copper-catalyzed cycloaddition of azide tagged bone marrow derived-MSC-EV to alkyne functionalized graphene oxide. This novel biomaterial offers the ability to combine the structural physicochemical benefits of graphene with the biological effects of MSC-EV. MSC-EV retain the intrinsic therapeutic properties of their parent cells and have shown to be effective in promoting tissue repair and regeneration, mitigating oxidative stress and modulating immune cell activities.
    The utility of MSC-EV as acellular therapeutics is being increasingly recognized. Their use is enhanced by several properties. Their cargo can be altered by exogenous loading to selectively enrich them with modulatory agents such as anti-sense oligonucleotides (George et al., 2018), miRNAs (Pomatto et al., 2019) or siRNAs (Matsuda et al., 2019). In addition, selective manipulation of their content is feasible through genetic engineering of donor cells to express RNA or proteins of interest. Moreover, their surface can be engineered to express specific markers that facilitate tissue- or cell-targeted delivery of the EV. Furthermore, their cellular production can be modulated by microenvironmental perturbations (Yan et al., 2017; Zhang et al., 2020). Of particular therapeutic relevance, MSC-EV also retain an ability to home to sites of inflammation and injury, similar to their parental cells (Lai et al., 2013). For all of these reasons, MSC-EV are attractive acellular therapeutics as well as therapeutic delivery vehicles with the capacity for targeted delivery of bioactive therapeutic molecules.
    [Theranostics is a term derived from a combination of the words therapeutics and diagnostics. In this emerging field of medicine, drugs and/or techniques are uniquely combined to simultaneously or sequentially diagnose and treat medical conditions.
    The ability to acquire a diagnosis and administer therapy in one package is a game-changer for medicine as we know it. Not only does this offer the opportunity to save time and money, but it also potentially allows one to bypass some of the undesirable biological effects that may arise when these strategies are employed separately.  
    Theranostics and Nanomedicine
    The medical application of nanotechnology is referred to as nanomedicine, and this is made possible by a vast range of medical and scientific methodologies. One such approach is the use of nanoparticles in theranostics. The ultimate aim of combining nanomedicine and theranostics is to modify disease and patient-specific outcomes in such a way that they are drastically improved.  https://www.news-medical.net/health/What-is-Theranostics.aspx ]
    The unique physicochemical properties and adaptability of graphene makes it attractive for development as a theranostic nanomaterial. Several biomedical applications such as drug and nucleic acid delivery, biosensing, photothermal, photodynamic therapy and tissue engineering have been proposed for graphene and its derivatives such as GO and rGO (Robinson et al., 2011; Hu et al., 2012; Lee et al., 2016; Shin et al., 2016; Vinothini et al., 2019). The oxygen-containing functional groups in GO and rGO contribute to their overall colloidal stability in aqueous solutions. These derivatives are often conjugated to polymers or other biomolecules in order to mitigate membrane-damaging effects or the effects of oxidative stress. Similarly, the conjugation of MSC-EV to GO may permit additional properties that can be exploited toward broader potential biomedical applications. The MSC-EV cargo contains a variety of bioactive molecules that can work alone, or in concert, to elicit a therapeutic effect (Liang et al., 2016; Yan et al., 2017). The modifiability of the EV cargo and the EV surface profile can contribute to achieve the desired biological effects in a targeted fashion (Ye et al., 2018). Furthermore, considering that EV and GO are internalized by different mechanisms, conjugation of EV to GO could enhance GO uptake by recipient cells (Huang et al., 2012; McKelvey et al., 2015). Thus, the biological effects of MSC-EV such as reducing tissue injury can be coupled with physical, biochemical or structural functionalities offered by graphene.

    An advantage of conjugation of EV to GO allows exploitation of the properties of graphene, such as surface modifications for additional functional properties. For example, cytotoxic effects of GO-EV could be augmented by loading chemotherapeutic that are released in a pH-responsive manner in tumor settings (Ardeshirzadeh et al., 2015; Wang et al., 2019). Other potential applications may involve fashioning the GO-EV as a structural biomolecule for implantation as an extracellular scaffold within tissues such as bone or teeth, or within endoprostheses and stents placed in the body (Diomede et al., 2018; Li et al., 2018). In this context, the ability to selectively load MSC-EV exogenously after isolation, or endogenously through genetic or protein manipulation of the parental cells offer the potential ability to use GO-EV as a therapeutic delivery platform. For such applications, further studies to determine the kinetics of EV release from GO-EV would be valuable to determine whether controlled release of MSC-EV can be accomplished for therapeutic benefit.
    The paucity of developmental or genotoxic effects of the GO-EV biomaterial paves the way for development of further applications in tissue engineering and regenerative medicine. Within the context of bone tissue engineering, GO elicits pro-osteoneogenic effects in vitro and in vivo (Hermenean et al., 2017). BM MSC-EV cargo has been shown to be enriched in several pro-osteogenic miRNAs (Ardeshirzadeh et al., 2015). Thus, GO-EV could augment the osteoinductive effects observed with GO. We observed variable effects on cytotoxicity of GO-EV and sGO-EV in liver cancer cell lines, though minimal cytotoxicity was observed in healthy hepatocytes. Notably, no genotoxicity was observed. Moreover, we did not detect any significant developmental toxicity in zebrafish. sGO-EV and GO-EV are readily recognized and phagocytosed by macrophages. Following their internalization by RAW264.7 cells, an alteration in the secretome profile with enhanced secretion of the pro-inflammatory cytokine, TNF-α was observed. The immunological impact of these biomaterials warrants further evaluation. Biocompatibility in vivo could be improved via the conjugation of GO with polymers that are capable of being cleaved upon internalization of the biomaterial to prevent the adverse accumulation of GO in cells (Li et al., 2014). Additional surface modifications may further reduce undesirable immune effects observed in our in vitro study.
    This study developed a process for fabrication of a graphene-based biomaterial incorporating MSC-EV and examined their cytotoxicity and immunologic effects in vitro and developmental toxicity effects in vivo. GO-EV induced an inflammatory response and cell-specific cytotoxicity. While some developmental malformations were observed, these had a minimal impact on overall survival in zebrafish. There are opportunities to further improve the biocompatibility of GO-EV. For example, variable effects of cytotoxicity have been observed with GO in different study settings. Cytotoxicity can be influenced by the flake size and the degree of oxygenation of GO, with the smaller and more oxygenated forms of GO eliciting more potent cytotoxic effects (Pelin et al., 2017; Gurunathan et al., 2019). Differences in GO induced cytotoxicity have been observed between different malignant and non-malignant cells (Fiorillo et al., 2015). Attention to optimized approaches and selection of base materials is warranted in future studies because cytotoxic effects could be impacted by the physical differences in the lateral dimensions and overall shapes of graphene noted between top-down and bottom-up synthetic approaches (Lee et al., 2019). Such efforts are warranted to take full advantage of the use of GO-EV as a functional biomaterial that combines the versatility of graphene with the intrinsic therapeutic effects of cell derived EV for the development of biomedical applications.
    Data Availability Statement
    The original contributions presented in the study are included in the article/Supplementary Material, further inquiries can be directed to the corresponding author/s.
    Ethics Statement
    The animal study was reviewed and approved by the Mayo Clinic Institutional Animal Care and Use Committee.
    https://www.frontiersin.org/articles/10.3389/fbioe.2021.686510/full

  18. Graphene Flagship

    Funded by the European Commission, the Graphene Flagship aims to secure a major role for Europe in the ongoing technological revolution, helping to bring graphene innovation out of the lab and into commercial applications.

    https://graphene-flagship.eu/

  19. Toxicity of graphene-family nanoparticles: a general review of the origins and mechanisms
        •    Lingling Ou,
        •    Bin Song,
        •    Huimin Liang,
        •    Jia Liu,
        •    Xiaoli Feng,
        •    Bin Deng,
        •    Ting Sun &
        •    Longquan Shao

    … several typical mechanisms underlying GFN toxicity have been revealed, for instance, physical destruction, oxidative stress, DNA damage, inflammatory response, apoptosis, autophagy, and necrosis …

    GFNs can be delivered into bodies by intratracheal instillation [30], oral administration [31], intravenous injection [32], intraperitoneal injection [33] and subcutaneous injection …

    GFNs can induce acute and chronic injuries in tissues by penetrating through the blood-air barrier, blood-testis barrier, blood-brain barrier, and blood-placenta barrier etc. and accumulating in the lung, liver, and spleen etc.  …

    … graphene nanomaterials aerosols can be inhaled and substantial deposition in the respiratory tract, and they can easily penetrate through the tracheobronchial airways …

    The toxicological mechanisms of GFNs demonstrated in recent studies mainly contain inflammatory response, DNA damage, apoptosis, autophagy and necrosis etc. …

    GO can result in acute inflammation response and chronic injury …

    Fibrosis and inflammation …

    The GFNs exert different toxicological effects on male or female reproductive system. pregnant mice had abortions at all dose, and most pregnant mice died when the high dose …

    The developmental toxicity of GFNs may induce structural abnormalities, growth retardation, behavioural and functional abnormalities, and even death. …

    The cytotoxicity of GFNs in vitro has been verified in various cells to change the cell viability and morphology, destroy the membrane integrity, and induce DNA damage [110–112]. GO or rGO decrease cell adhesion; induce cell apoptosis; and enter lysosomes, mitochondria, cell nuclei, and endoplasm [113]. GQDs entered cells and induced DNA damage …

    Graphene can increase cell viability [117] or cause cell death …

    … cause dose-dependent toxicity … The high content of GO mainly deposited in the lungs, liver, spleen, and kidneys and was difficult to be cleaned by the kidneys …

    Increasing concentrations of GO entered the lysosomes, mitochondria, endoplasm, and cell nucleus …

    GO can insert between the base pairs of double-stranded DNA and disturb the flow of genetic information at the molecular level, which might be one of the main causes of the mutagenic effect of GO …

    … the importance of the GO surface charge because of its ability to affect the internalization and uptake mechanism of cells …

    … strong electrostatic interactions between the negatively charged oxygen groups on the GO/GS surface and positively charged phosphatidylcholine lipids on the RBC outer membrane …

    The physical interaction of graphene nanoparticles with cell membranes is one of the major causes of graphene cytotoxicity …

    … the sharpened edges of GNS may act as ‘blades’, inserting and cutting through bacterial cell membranes …

    … overwhelm the activity of antioxidant enzymes, including catalase, SOD, or glutathione peroxidase (GSH-PX) … The interactions of GO with cells can lead to excessive ROS generation, which is the first step in the mechanisms of carcinogenesis, ageing, and mutagenesis …

    Exposure to GFNs resulted in significantly increased coupled and uncoupled mitochondrial oxygen consumption, dissipation of the mitochondrial membrane potential, and eventual triggering of apoptosis by activating the mitochondrial pathway …

    GFNs can cause apoptosis and/or cell necrosis by direct influencing cell mitochondrial activity …

    Due to its small size, high surface area and surface charge, GO may possess significant genotoxic properties and cause severe DNA damage, for example, chromosomal fragmentation, DNA strand breakages, point mutations, and oxidative DNA adducts and alterations …

    Even if GO cannot enter into the nucleus of a cell, it may still interact with DNA during mitosis when the nuclear membrane breaks down, which increases the opportunity for DNA aberrations …

    The π stacking interaction between the graphene carbon rings and the hydrophobic DNA base pairs can make a DNA segment ‘stand up’ or ‘lay on’ the surface of graphene with its helical axis perpendicular or parallel, respectively. The intermolecular forces severely deform the end base pairs of DNA, which potentially increases the genotoxicity …

    DNA damage can not only initiate cancer development but also possibly threaten the health of the next generation if the mutagenic potential of GO arises in reproductive cells, which impacts fertility and the health of offspring …

    A strong inflammatory response was induced by subcutaneously injection …

    Apoptosis is defined as the self-destruction of a cell regulated by genes through complicated programmes … after inhalation …

    … graphene and GO physically damaged cell membranes … increased the permeabilization of the outer mitochondrial membrane and changed the mitochondrial membrane potential … triggered by the death-receptor and canonical mitochondrial pathway …

    Necrosis is an alternate form of cell death induced by inflammatory responses or cellular injury. The exposure of cells to pristine graphene causes apoptosis and necrosis at high doses …

    GFNs could cause subtle changes in gene expression programming by modulating epigenetic changes.

    https://pubmed.ncbi.nlm.nih.gov/27502632/

    1. This is madness, in the quest to create their Internet of Things, with controllable Human cyborgs being just another Thing, they are seriously contaminating the Earth…I wonder what Enki thinks of this ?

    2. So, the internet of things, is worth all of our lives?  Or are we supposed to become part of the Borg?  Either way, they are screwing us all up.  Why because of communication junkies?  Or they are too lazy to wait for information to download? 

      I fail to understand why people think this ok.  It's not ok!  ok!

  20. I'm guessing that they probably used Graphene for this engineered winter weather event, to likely destroy the world's largest coffee bean crops. IN BRAZIL?

    Photos show snow on Brazil streets during a rare winter weather phenomenon (msn.com)

    "Some Brazilians tweeted their reaction on the winter phenomenon, saying the snowfall brought 'intense cold air-break' and hail."

    Sounds exactly like the intense cold air-break and hail phenomenon we had here in Texas back in February!

    Ironic how most of these engineered winter weather and heat dome events, only seem to occur where crops, vineyard, orchards and other agricultural based economies are located. Or major gas, coal and oil refineries are operating. Plus the "mystery" cyber attacks on pipelines and meat processing plants.

    Anyone else seeing a pattern developing here, besides just Dane? 

    Modern and historical warfare tactics include crippling the civilian populations and I find it hard to believe that Nature would be just as discerning. Because the military industrial complex continues to grow stronger with each passing weather (Climate) anomaly. While the general public starves, freezes or suffers from heat stroke!

    In their book, all of this is considered "Mission Accomplished". Especially when it can all be done covertly, while operating right out in plain sight as well.

  21. Thank you Dane. I mentioned to you on a You Tube post I'd been smelling aluminum in my sinuses on heavily cloudy days. Now I know why.

    It's too bad more of my acquaintances can't seem to wrap their heads around what you've been informing me about for years.

  22. I was wondering why the skies look different as of late. They spray very early now in the morning. It is stringy clouds and they burn off by midday. There is still a lot of aluminum because I see it sparkling in sunlight. Graphene, wow, our world covered in it and our bodies injected with it. You cannot make this evil up!

  23. Thank You Dane!!! This absolutely confirms what I've been telling others about viruses amidst the other toxins in the spraying. It's even effected my little dog as she has developed a chronic cough that gets worse when we've spent time outdoors.

    I was able to watch this on my Roku TV 'You Tube' App but not on my iPhone You Tube App. That App is playing every other video with similar titles and wording but not showing this video. APPLE is definitely not getting positive feedback on this, from me! I hope it stays on You Tube for everyone to watch!

    Thank You again for this Vital Information Update!!!

    RandyJ

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